Fayyaz Rasool, Shafat Hussain and Shakeela Parveen
Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore-Pakistan
Labeo rohita is a commercially important fish of South Asia. A study on the variation amongst the different populations of on the basis of ATPase 6/8 gene was conducted. The present study evaluates the potential of complete ATPase 6/8 region of mitochondrial DNA as a marker region to determine the phylogeography of Labeo rohita from fish farm of region Punjab, Pakistan. mtATPase6/8 (878 bp) regions was used to investigate genetic variation within Labeo rohita and develop a global genealogy of genus Labeo strains. The mtATPase6/8 region was more variable but the given the wide distribution of Labeo rohita the overall levels of sequence divergence were low. Levels of haplotype diversity varied widely among countries with Chinese and India showing the greatest diversity whereas Japanese Labeo had undetectable nucleotide variation. . Chinese and Japanese carp strains were the most divergent, and their relationships do not support the evolution of independent Asian and European lineages and current taxonomic treatments. The results revealed that 878 bp of ATPase 6/8 region could be a promising marker for determining variations at interpopulation as well as intrapopulation levels in Labeo rohita. These results would facilitate conservation and management of this important species
Keywords: ATPase6/8, Variation, Populations, Labeo rohita.