Katsuhiro Shiratake, Mami Suzuki, Hiroshi Sakamoto, Moeko Taki, Ryo Nakabayashi, Yoichiro Fukao, Yoshiyuki Ogata, Nozomu Sakurai, Toshiaki Tokimatsu, Susumu Goto and Kazuki Saito
Graduate School of Bioagricultural Sciences, Nagoya University, Japan
Grape is an important crop as a horticultural crop and for wine production. Grape accumulates different polyphenolic compounds, such as anthocyanin, catechin and resveratrol, in the berry skin. These compounds decide color and taste of fruit and wine and are known as beneficial compounds for human health. We performed transcriptomics and metabolomics of grape berry skin after ultraviolet irradiation, and proteomics and metabolomics of grape culture cells after light irradiation or elicitor and jasmonic acid treatment. The metabolic pathway maps projected the obtained omics data clearly show specific induction of anthocyanin or resveratrol synthetic pathway by different stimuli. Proteomics of microsomal fraction revealed known anthocyanin transporters and putative ones. We will discuss the possibility of anthocyanin and resveratrol productions using the grape culture cells with metabolic engineering, too.
Keywords: Grape, anthocyanin, resveratrol, omics, culture cell.