Tang Jinli, Xiaoming Wang, Ge Mengyuan, Gui Zhen, Li Jinchang, Zhu Mingchen and Yan Feng
Department of ClinicalLaboratory, Nanjing MedicalUniversity Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing 210009, China
Objective To explore the relative expression of plasma miR-199a-5p and miR-200c-3p in gastric adenocarcinoma cancer(GAC) patients and its clinical value.
Methods: Case-control studywasused in this research. The relative expression of plasma miR-199a-5p and miR-200c-3p from 47 GAC patients and 50 healthycontrolsweredetermined by RT-PCR(TaqMan Probe method). Meanwhile, the association with age, gender, tumor location, size, degree of differentiation, TNM stage, lymph node metastasis and other clinical pathological parameters were analyzed. The expression of thesetwomiRNAs in plasma of 30 GAC patients duringpreoprationwascomparedwiththeir expression 6-8 days after radical surgery. The sensitivity and specificity of plasma miRNAs expression for the diagnosis of GAC were analyzed using the receiver operating characteristic (ROC) curve. SPSS20.0 statistical software was used for statistical analysis. T-test, paired t-test and one-factor ANOVA were used for normal distribution of quantitative data.
Results: The plasma level of miR-199a-5p in GAC patients was significantly lower(1.05±0.22) (t=3.058,P=0.003), while miR-200c-3p was significantly higher(15.15±3.02)(t=-2.854,P=0.006), when they were compared with those in controls(26.80±8.38, 3.39±0.87). Low miR-199a-5p expression in GAC patients were associated withlymphnodemetastasis(F=4.725,P=0.029) and the differentiationdegree of gastric cancer(F=3.854,P=0.032). The relative expression of miR-199a-5p in postoperative plasma wassignificantlyincreased(t=-3.814,P=0.001), but the relative expression of miR-200c-3p was significantly reduced when compared to the preoperative samples(t=2.978,P=0.006). Area under the ROC curve of miR-199a-5p, miR-200c-3p and combined miR-199a-5p and miR-200c-3p were 0.692, 0.792 and 0.798, the sensitivity and specificity were 87%,97%,92.5% and 43%,54%,65%, respectively.
Conclusion: Combineddetection of miR-199a-5p and miR-200c-3p in plasma has a higher sensitivity and specificity than the conventional tumor marker CEA and CA19-9. and may be a useful biomarker for gastric cancer diagnosis.
Keywords: Gastricadenocarcinoma cancer, plasma microRNAs, tumor markers, real-time fluorescent quantitative PCR.