Bover, E. A, Sanchez, D.A, Belda, M.A, Padrón, C. A, Cejalvo, D.A and Lloris, J.M.B
Facultad de Ciencias Experimentales, Universidad Católica de Valencia “San Vicente Mártir”, C/ Guillem de Castro, 94. 46003. Valencia, Spain
Seaweeds have developed strong antioxidant systems in response to highly oxidative conditions in which they live. They are considered to be a rich source of antioxidants and have been identified to possess biochemical substances with therapeutic potential [1]. Phenolic antioxidant compounds have gained importance due to their high demand for alternative and safe antioxidants from natural resources. Antioxidant agents have been used to prevent tissue damage in various clinical settings and experimental models and could help in preventing the post-ischemic increases in lipid peroxidation and hydrogen peroxide levels, resulting in reduced IR injury [2, 3].
Therefore, the green seaweed Caulerpa prolifera, was evaluated for its Total Phenolic Content (TPC) and for its Radical Scavenging Activity (RSA). The effect of variables such as: solvent, temperature and time, have been studied to optimize the polyphenol extraction.
C.prolifera showed the maximum TPC and RSA in ethanol at 3 h 45°C. Identification and quantification of phenolic compounds was carried out with RP-HPLC/DAD. Biochemical analyses were carried out (MDA, catalase, Gpx, SOD and LDH), protein analyses (cytokines and proinflammatory molecules), Western Blot and the histologies to determine the intestinal morphology and the physiopathological changes during the period of damage due to IRI were also characterized.
REFERECNES
[1] Monsur-Hammed A, Tope Asiyanbi-Hammed T, Jaswir I, Amid A, Zahangir Alam Md. Effects of drying methods on nitric
oxide inhibition potential of water soluble extracts of Turbinaria turbinata : A brown seaweed species of Malaysian origin.
American Journal of Drug Discovery and Development. 2013; 3(4): 279-285.
[2] Kim J, Kil IS, Seok YM, Yang ES, Kim DK, Lim DG, Park JW, Bonventre JV, Park KM. Orchiectomy attenuates postischemic
oxidative stress and ischemia/reperfusion injury in mice. A role for manganese superoxide dismutase. J Biol Chem.
2006; 281: 20349-20356.
[3] Prieto-Moure B, Caraben-Redano A, Aliena-Valero A, Cejalvo D, Toledo AH, Flores-Bellver M, Martinez-Gil N, Toledo-
Pereyra LH, Lloris Carsi JM. Allopurinol in Renal Ischemia. Journal of Investigative Surgery. 2014; 27: 304-316.