Hala El-Adawi, Mahmoud Taha, Abdel Fattah Abdel Fattah and Nagwan Youssef
Medical Biotechnology Dept., Genetic Engineering & Biotechnology Institute, P.O.BOX 21934, Alexandria, Egypt
C. albicans induces infections of different kinds, classified as superficial, locally invasive and systemic infections. The present study aimed at estimating the antifungal activity of Lactic acid bacteria (LAB) extract against 30 resistant clinical isolates of the vegetative form of candida albicans (C. albicans).LAB was isolated from 100 fermented food samples. C. albicans isolates and their biofilm were obtained from private hospitals. The different isolates species of LAB extracts were then evaluated for its antimicrobial activity on C. albicans isolates by using agar well diffusion method. Determination of MIC of LAB extracts were then carried out. Moreover, the effect of LAB extract on the formation of C. albicans biofilm was assessed and the inhibition was then expressed as percentage.
All LAB extracts exerted a growth inhibitory effect on the vegetative form as well as the biofilm of C. albicans and the most powerful isolate was L. plantarum , where, the highest diameter of inhibition zone was 22.80mm and the MIC was 62.67% while the average percentage of biofilm inhibition was 67.33%.The use of probiotic LAB strains may offer an alternative therapeutic choice in the treatment of Candidiasis and effectiveness against drug resistant strains since all LAB extract proved inhibition.