Nuraly Akimbekov, Abdieva Z. Gulzhamal, Ualyeva S. Perizat, Kaiyrmanova K. Gulzhan, Digel Ilya and Zhubanova A. Azhar
Department of Biotechnology, Al-Farabi Kazakh National University, Kazakhstan
Microbial cells immobilized on solid surfaces cover nowadays a wide area of applications in biotechnology. A unique combination of valuable physical and chemical characteristics makes nanostructured carbonized sorbents of plant origin very attractive as a constituent of heterogeneous composite systems containing microbial cells. Due to their remarkable properties, nanostructured carbon rice husks (CRH) can be used as sorbents for adsorption of different industrially important microorganisms. Our research is aimed to creation of cost-effective and sustainable bio-composite materials on the basis of microbial cells adsorbed CRH. Electron microscopy studies confirmed that multiple probiotic and protein producing valuable cells can successfully attach, survive and proliferate inside the porous network of the CRH. In our model experiments, the carbon material specifically adsorbed up to 95% microbial cells from various solutions. The resulting biocomposite materials possess outstanding probiotic and nutraceutical properties accompanied by high specificity, depending on the particular microbial strain used. The in vivo and in vitro studies strongly suggest that the use of the CRH as carrier for the oral administration of probiotic microorganisms has a very big potential for improving functionality and safety of probiotic preparations. This interdisciplinary knowledge could significantly stimulate development of novel immobilized biocatalysts possessing high activity, selectivity and stability.
Keywords: Nanobiocomposites, carbonized sorbents, immobilization.