Tae-Ho Park and Kwang-Soo Cho
Department of Horticulture and Institute of Life and Environment, Daegu University, Gyeongsan 712-714, Republic of Korea
Somatic hybridization has been widely applied to introgress several disease resistance from wild Solanum species into the cultivated potatoes (Solanum tuberosum) and plastome genotyping is one of important processes to select proper breeding lines. In this study, we completed chloroplast genome sequences of two wild Solanum species, S. commersonii and S. nigrum, which have been known and used as one of useful resources for improving resistance to several diseases. The sequences were compared with those of Capsicum annum, Nicotiana tabacum, S. lycopersicum, S. bulbocastanum and S. tuberosum to develop InDel markers for the application in cytoplasm genotyping. Although gene contents and their relative positions were almost same, detailed comparison of their sequences identified several indels between S. commersonii / S. nigrum and S. tuberosum in the intergenic and intragenic regions. Based on the sequence information of the indels, one and seven allele specific RCR-based markers were developed for discrimination of S. commersonii / S. nigrum from S. tuberosum and the markers were further confirmed with filial generations and other wild Solanum species, respectively. Additionally, the phylogenetic tree revealed S. commersonii is located in a same node with S. tuberosum, but S. nigrum was slightly further from the node.
Keywords: Chloroplast genome sequence, molecular marker, potato, Solanum commersonii, Solanum nigrum, Solanum tuberosum.