ETHANOL PRODUCTION USING RED BEET JUICE BY SACCHAROMYCES CERVISIAE ATCC 9763

Venegas-Sánchez Josué Addiel and Jiménez Islas Donaji

Huichapan Higher Technological Institute, Renewable Energy, El Saucillo, Huichapan, Hidalgo, Mexico

Abstract

In Mexico, red beet (Beta vulgaries L. conditiva) is available all the year, therefore its juice and bagasse can be used for bioethanol production. In this work was investigated the ethanol production by Saccharomyces cervisiae ATCC 9763 using juice red beet at pH = 2.8 and 37 °C. The rates of biomass growth, sugar consumption and ethanol production during batch fermentation of red beet juice were estimated. The logistic, Pirt, and Luedeking-Piret equations were used to model the microbial growth X(t), substrate consumption S(X), and ethanol production P(X), respectively. The volumetric ethanol productivity (Qp) and ethanol production (P) were 0.86 g L-1 h-1 and 28 g L-1, respectively. The maximum specific growth rate (μmax) and maximum biomass concentration (Xmax) were 0.23 h-1 and 1.95 g L-1, respectively. These results indicate that a significant portion of the carbon source was used for maintenance of strain, which agrees with the fact that the lowest values of Xmax obtained under these fermentation condition. The strain of Saccharomyces cervisiae ATCC 9763 utilized in this study was able to produce ethanol with high yield and volumetric productivity under acid and thermal stress condition.