ZHANG Tao, FAN Qing-can, ZHANG Xiang-qian, ZHANG Gen-xi, WANG Jin-yu and GU Yu-ping
College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou 225009, China
Body composition traits are important economic traits of chicken. To identify SNPs and candidate genes affecting body composition traits and provide new methods for genetic inprovement, a genomewide association study was carried out using the Illumina chicken 60K SNP Beadchip in Jinghai Yellow Chicken. Thirteen Body composition traits were measured. A total of 13 SNPs reaching 5% Bonferroni genome-wide significance (P<1.8E-6) and 130 SNPs reaching “suggestive” genome-wide significance (P<3.59E-5) with body composition traits were identified. Those SNPs were located nearby or in twelve candidate genes including GRIK1, NCAPG, KCNIP4, CACNA2D2 and so on. A region approximately 1.6 Mb in length on chicken chromosome 4 (74.3-75.9 Mb) was found to be associated with body composition traits of Jinghai Yellow Chicken. Among 130 SNPs reaching “suggestive” significance, 25 SNPs were located in a region 7.4 Mb in length on chicken chromosome 4 (71.5-78.9)._5,650 haplotpyes were established and fourteen of them were found to be associated with six body composition traits. Nine out of fourteen haplotypes were located in the region of 74.3-75.9 Mb on GGA4. Five candidate genes of LCORL, QDPR, KCNIP4, LDB2 and FAM184B were located in this region. The present study indicated that the region of 71.5-78.9 on GGA4 and GRIK1, NCAPG, KCNIP4, CACNA2D2, LCORL, QDPR, KCNIP4, LDB2, FAM184B gene might paly important role in regulation of body composition of Jinghai yellow chicken.