Chetana Roat and Shailesh R. Dave
Microbiology and Biotechnology, School of Sciences,Gujarat University, Ahmedabad, India
Resveratrol and Viniferin possess a broad spectrum of pharmacological and therapeutic effect like anti-cancer, antioxidant, anti-atherosclerosis. Endophytes, by residing within the specific chemical environment of host plant tissue, form unique group of microorganisms. Microbially unexplored medicinal plants can have diverse and potential microbial association. The aim of this work is to identify and isolate the endophytic microorganisms that possess resveratrol and viniferin producing capability and optimizes the conditions for their production. Endophytes from Vitis vinifera L.cv. Merlot, Vitis quinquangularis Rend. and Cayratia trifolia L. were studied and the resveratrol and viniferin producing isolates were screened by thin Layer Chromatographic (TLC) with solvents system CHCl3/MeOH/HCOOH, 85/15/3 (v/v) and identification of the compound was done by HPLC. The total of 20 isolates were obtained, 5 were endophytic bacteria and 15 were endophytic fungi and it was found that the one strain Aspergillus sp. AB4 isolated from leaf of Vitis vinifera using Czapek yeast extract agar medium had stable resveratrol and viniferin producing capability in all subcultures. Cell growth of Aspergillus sp. increased during cultivation and reached a stable and high level of biomass after 7 days. The best fermentation conditions for resveratrol and viniferin production in broth cultures of Aspergillus sp. AB4 were an inoculum size of 10%, a rotation speed of 100 rpm , and a temperature of 28°C.
Keywords: Resveratrol, Viniferin, Endophytes, Aspergillus, HPLC.