THE EFFECT OF DIFFERENT MOLECULAR WEIGHT COLLAGEN PEPTIDES ON MC3T3-E1 CELLS DIFFERENTIATION

Junli Liu, Shaoyan Si, Yaya Qin, Bin Zhang, Shujun Song and Yanchuan Guo

Key Laboratory of Photochemical Conversion and Functional Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, People’s Republic of China

Abstract

Our study aimed at investigating the effect of different molecular weight bovine collagen peptides, namely CH878, CH1370, CH2900, and CH7747 on the differentiation of MC3T3-E1 cells. Osteogenic differentiation of MC3T3-E1 cells was assessed by a series of specific assays, after culturing of cells in the presence of collagen peptides. Alkaline phosphatase activity (ALP) was evaluated by NBT/BCIP staining. Osteocalcin expression was determined by a radioimmunology method. Mineralization was quantified by Alizarin Red staining. ALP staining results demonstrated that the ALP staining of cells after culture in the presence of collagen peptides were significantly higher than the control group (P＜0.05), indicating the promotion of ALP activity in MC3T3-E1 cells by these peptides. Radioimmunology results demonstrated that collagen peptides groups were all significantly higher than the control group (P＜0.01). Alizarin Red staining results demonstrated that CH1370, CH2900, and CH7747 significantly promoted the formation of mineralized bone matrix. We therefore conclude that CH1370, CH2900, and CH7747 play an active role in the differentiation of MC3T3-E1 cells. Based on the above results, we provide molecular basis for further development of collagens with different molecular weight for the prevention and treatment of osteoporosis.