PEG CAPPED METHOTREXATE SILVER NANOPARTICLES FOR EFFICIENT ANTICANCER ACTIVITY AND BIOCOMPATIBILITY

Zarmina Muhammad, Sundus Riaz, Syeda Sohaila Naz and Nosheen Fatima Rana

Department of Biomedical Engineering, National University of Sciences and Technology, Islamabad, Pakistan

Abstract

Nanocarriers endow tremendous benefits to the drug delivery systems depending upon the specific properties of either component. These benefits include, increase in the drug blood retention time, reduced efflux, additional toxicity and targeted delivery. Methotrexate (MTX) is clinically used for cancer treatment. Higher dosage of MTX results in hepatic and renal toxicity. In this study methotrexate silver nanoparticles (Ag-MTX) coated with polyethylene glycol (PEG) are synthesized and characterized. Their anticancer activity and biocompatibility is also evaluated. RESULTS: Ag-MTX nanoparticles are synthesized by chemical reduction method. They are characterized by Ultraviolet-Visible Spectroscopy and Fourier Transform Infrared Spectroscopy. Average size of PEG coated Ag-MTX nanoparticles (PEG-Ag-MTX nanoparticles) is 12nm. These particles exhibited improved anticancer activity against MCF-7 cell line. Hemolytic activity of these particles was significantly less than MTX. CONCLUSION: PEG-Ag-MTX nanoparticles are potential nanocarrier of methotrexate which may offer MTX based cancer treatment with reduced side effects. In-vivo investigations should be carried out to explore them in detail.

Keywords: Anticancerous drug; Chemotherapeutics; Hemocompatibility; Methotrexate; Pegylation; Silver nanoparticles.