LUTEAL PHASE OF PORCINE FOLLICULAR FLUID SECRETION EFFECT ON PRIMARY OVIDUCTAL EPITHELIUM CELL

Natthason Buranathanasin

Department of Biology, Faculty of Science, Silpakorn University, Nakorn Pathom, Thailand

Abstract

Effect of luteal phase porcine follicular fluid (pFF) from medium size (4-6 mm diameter) on primary porcine oviductal epithelial cell (POEC) viability was evaluated by MTT assay and analyzed by one-way ANOVA. POEC were cultured in M199 supplement with 10% fetal calf serum (FCS) in high humidity with 5% CO2 at 37°C for 96 h (n=6). After culture time, POEC was spindle shape and expansion. Then, they were treated with pFF from medium size follicle at concentration of 2 μg, 4 μg, 20 μg, 40 μg, 200 μg, 400 μg, and 500 μg, for 24 h. We found percentage of cell viability at concentration of 2 μg, 4 μg, 20 μg, 40 μg, 200 μg, and 400 μg showed slight increase in cell viability. Meanwhile, percentage of cell viability at 500 μg was exhibit higher than positive control (medium with 10% FCS+ hormone) and control group but not significant (p>0.05). Even though this finding is basic research, but the results would be important for use pFF as replacement of FCS and hormone in culture medium, in the future study.

Keywords: Primary cell culture, oviductal epithelium cell, follicular fluid, MTT assay, porcine.